This test will determine if two or more males are from the same male lineage. This test is often used to indirectly establish paternity when the alleged father is not available. Journey Genetic Testing.

Y Chromosome Test (Y-DNA)

Y-Chromosome Male Lineage DNA Test – $159

What is a Y-Chromosome DNA test?

The Y-Chromosome DNA test, sometimes referred to as a Y-STR comparison, is a test that can be performed between two males to determine if they share the same male ancestral line. It is often used in paternity cases where the alleged father is not available for testing. This test can indicate whether or not a child is related to the alleged father’s brothers or other male relatives who share a common paternal line. It can also be done between alleged two or more alleged half-brothers. The DNA of the mother is not needed when this option is used to determine paternity.

A  Y-STR analysis cannot distinguish a specific relationship between males who belong to the same paternal line. However, it is very useful for excluding males from an alleged biological relationship. For example, if a male child and his alleged uncle (alleged father’s full brother) are tested, their Y-STR profiles must match. If they do not, then the alleged uncle is excluded (not considered a biological uncle) and the brother of the uncle tested is probably not the biological father.

How does a Y-Chromosome DNA test work?

Y- STR is an abbreviation for Y Chromosome Short Tandem Repeats (STRs) which are found on the male-specific “Y” chromosome. The coding genes, mostly found on the short arm of the Y-chromosome, are vital to male sex determination and other male related functions. Based on the genetic fact that the Y-chromosome is passed from father to son(s) relatively unchanged through many generations and has an infrequent mutation rate, this DNA test examines highly differential markers on the Y chromosome and DNA profiles for these markers are generated. These Y-STR profiles are then compared to determine if there is a match. Males related through their fathers will have the same or very similar Y-STR profiles, and those who are not have different profiles.

Although the Y-Chromosome is only found in males, women can also indirectly participate in a Y-STR DNA test if they are interested in determining their paternal relatives. They would need to ask a biological male relative, such as a father, brother, paternal uncle, or paternal grandfather to contribute a sample for comparison testing with her potential paternal relative.

How accurate is a Y-Chromosome test?

This test is based on the genetic profiles found in the Y-Chromosome of the males testing. It is 100% accurate showing either that the two males do belong to the same male line, meaning they are related through a common paternal relative, or an exclusion showing they do not share the same male line. A Y-Chromosome test will not identify the specific relationship of those testing, however.

Male Lineage DNA Sample Collection

The most accurate method of obtaining DNA for an Y-STR DNA test is to use a buccal (mouth) swab, which is very similar to a “Q-tip” cotton swab. The swabs are rubbed on the inner cheeks of the mouth of those being tested.  The process is simple and fast. We provide all the materials needed for the DNA collection in our Journey Genetic Testing Test Kit.

However, we know that sometimes it is not possible to obtain a buccal swab sample. We are often able to test discreet alternative DNA samples. To learn more about non-standard, alternative DNA samples that we can test, CLICK HERE.

Your DNA collection kit will have detailed step-by-step instructions on how to collect your swab sample. You can also read more about how to take a home DNA test by going to How To Collect A DNA Sample At Home.

If you need to send one or more of the DNA collection kits to a different address, we can do that as well. Just indicate how may collection kits you need when you complete the order form, and enter the addresses for each kit in the area of the form where it asks where the kits are being sent.

What if those being tested live in different cities?

A Y-Chromosome DNA test can be easily conducted even if the different parties to be tested are in different locations. Each DNA testing kit is assigned a unique identification number. If each person lives in different cities, or different countries, Journey Genetic Testing DNA Test Kits with the unique case number can be sent directly to each person. After the DNA collection, the DNA samples can be sent separately to the laboratory.

The DNA specimens will be correctly matched and processed once all sample have arrived. When ordering online, select the option of two kits, or more depending on the number of people being tested, on the order form and enter the additional name and address in the box that will automatically show when this selection is made!

Making Your Own DNA Kit (MYOK)

All DNA test orders include a DNA collection kit sent to the designated address on the order form. We understand that you may be in a situation where you do not want anything being mailed to you, to allow additional privacy.

Journey Genetic Testing allows you the option to make your own testing kit when collecting and returning DNA samples to us. This option can save money on shipping a DNA kit to you,  as well as save you time as you can ship directly to us  without waiting for the testing kit to arrive.

To choose this option please select the “Make My Own Kit” option on the order form for the particular test you are seeking. You will find the order form on the information page for that particular test. For example, paternity test order forms are found on the paternity page, sibling order forms are found on the sibling page, and so on.

Upon finalization of your order (during regular business hours) you will be e-mailed a submission letter with your unique Journey Genetic Testing case number and the address for mailing your samples. The processing of the samples is conducted in the same time frame as if you used one of our kits – in as little as 1 business days for paternity, maternity, sibling, grandparent, avuncular, and twin zygosity tests!

Non-standard samples being submitted must fit into a standard envelope. If necessary please trim the item to fit (for example a hat band, a piece of clothing with blood, etc) into a regular envelope while making certain the specific area to be tested is included.


It is strongly suggested that if you reside outside of the United States and wish to order DNA testing that you use this option as the most cost effective and time efficient method to get the answers you need and deserve.

Please note that we are unable to process any DNA samples without a Journey Genetic Testing case number. Samples that arrive without a case number will be discarded.

To learn more about the Make Your Own Kit option, please CLICK HERE.

Since every male from the same paternal blood line have the same Y chromosome, the results of a Y-STR DNA test cannot tell you the specific relationship between the males tested. You will need to draw your own conclusions based upon the result of the test and what you are attempting to determine.

  • Y chromosome testing can be used to establish indirect paternity when the alleged father is unavailable to test, or will not submit to the test.  In these situations, we can test a direct male relative of the alleged father with the DNA of another male to confirm whether the untested alleged father is in fact related to the person claiming to be a biological relative.
  • If can be used when two siblings wishing to confirm whether or not they have the same biological father can do so by doing a Y chromosome test. If there is a possibility that both alleged fathers are related, then either a sibling test, or direct paternity test, is recommended.
  • Adopted children who are searching for their real biological fathers, and perhaps have found a possible male family member,  can use a Y chromosome test in order to include, or exclude, potential fathers or paternal relatives.
  • Legal Y chromosome testing can sometimes be used in cases requiring legal proof of relationship between males such as probate or inheritance cases, or DNA proof for immigration.

No. Unlike a mtDNA test where the mother passes mitochondrial DNA to all her children, only a male child would inherit the Y-Chromosome from his biological father. Since females do not have the Y-Chromosome, this test cannot be used in these cases.

Y chromosome testing is used in criminal forensic investigations and cases involving rapes. If a genetic sample is found at a crime scene, testing for the Y chromosome can establish whether there is any male DNA in the sample. Once the Y chromosome has been confirmed, it can be compared to the Y chromosome profiles of suspects to confirm a match.

How much does a Y-Chromosome test cost?

Journey Genetic Testing is able to perform the most accurate Y-Chromosome DNA testing available, comparing 22 specific genetic markers on the Y-Chromosome. All pricing is determined by the number of males being tested and the type of sample being sent.

When ordering a Y-STR test you may choose to either use a testing kit from Journey Genetic Testing or have the option to make your own DNA collection kit. This may be especially helpful if you need to have non-swab samples tested, or do not want to wait for the DNA kit to arrive to your shipping location if you are facing time constraints to obtain results.

Number Of People Testing Lab Processing Time Cost
Two males 3-5 business days $159
Three males 3-5 business days $269
Four males 3-5 business days $337
Non-standard DNA sample (each) +5 business days $125

Example of Y-Chromosome Male Lineage Test Results

Y Chromosome DNA Test ResultA Y-STR report is one of the most direct, and easy to understand, DNA tests. The results report will show the genetic markers that have been tested, and will provide the summary of the findings at the bottom of the page. There will only be one of two results listed:

  1. The genetic evidence show the male donors share the same male lineage, meaning the are related through the paternal line.
  2. The genetic evidence excludes the make donors from being related through the same male line.